Welcome!
ALERT ATTENTION ⚠️
On Saturday April 20th, 2024 at 8:00 AM (UTC), emergency site maintenance has been scheduled for iLab Primary U.S. Details and updates can be found on the Agilent Status Page. For additional information, please contact the Agilent iLab Support by clicking on 'Help' at the top of your account page or by emailing ilab-support@agilent.com.
This facility serves the needs of SKCC research programs in structural biology, including cryo-electron microscopy, crystallization, x-ray data collection, structure determination, and structural analysis of macromolecules. The facility has a ThermoScientific 200 kV Glacios transmission electron microscope equipped with a Falcon 4 direct electron detector, a Vitrobot 5 for cryo-EM sample preparation, a Formulatrix NT8 robot crystallization robot for high-throughput crystallization screening and maintains a Rigaku MicroMax-007HF microfocus rotating anode X-ray generator dual equipped for data collection from macromolecular crystals and Small Angle X-Ray Scattering (SAXS). The left port of this instrument, dedicated to X-ray data collection from macromolecular crystals, is equipped with high focusing mirrors and a new ultrafast PILATUS 200K Hybrid Pixel Array Detector and a X-stream 2000 cryogenic system, while the right port hosts a new BioSAXS-2000 2D Kratky SAXS system.
The facility also provides access to instrumentation for biophysical characterization of proteins, and protein/protein and protein/DNA interactions. These instruments include a Beckman Coulter Analytical Ultracentrifuge (ProteomeLab XL-I/A Protein Characterization System), a JASCO J-810 Spectropolarimeter, a BIACORE 3000 SPR biosensor, and a TA Instruments Nano-Isothermal Titration Calorimeter.
Dmitry Temiakov, Ph.D | Director
Erik Debler, Ph.D. | Co-Director
Ashok Nayak, Ph.D | Cryo-EM Facility Manager
Hideharu Hashimoto, Ph.D. | X-ray Crystallography Facility Manager
| Hours | Location |
|
Staffed Monday - Friday, 9am - 5:00pm 24/7 access for trained users |
Glacios Control room: JAH 444 Vitrobot/ITC: JAH 442 X-ray/CD: JAH 457 1020 Locust Street 19107 Philadelphia PA
|
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Dmitry Temiakov, PhD |
Director
|
(215) 503-4692
|
Dmitry.temiakov@jefferson.edu
|
JAH 425e
|
| Erik Debler, PhD |
Co-Director
|
(215) 503-4596
|
Erik.debler@jefferson.edu
|
JAH 411f
|
| Ashok Nayak, PhD |
Cryo-EM Manager
|
(215) 503-4030
|
Ashok.nayak@jefferson.edu
|
JAH 425c
|
| Hideharu Hashimoto, PhD |
X-ray crystallography Manager
|
(215) 503-9252
|
Hideharu.hashimoto@jefferson.edu
|
JAH 412
|
https://goo.gl/maps/QdEEHgUXwXE2
