SKCC Bioimaging Facility Shared Resource

Mission

The goal of the Sidney Kimmel Cancer Center Bioimaging Shared Resource is to advance the scientific research programs of the Cancer Center by providing powerful, reliable, and readily accessible light microscopic image acquisition and analysis capabilities to SKCC investigators. The Resource provides a state of the art STED (stimulated emission depletion) super-resolution system, laser point-scanning and spinning disk confocal, TIRF (total internal refection fluorescence), and widefield epifluorescence microscopy capabilities allowing for multi-wavelength visualization and analysis of fixed and living specimens, Z series, single molecule events at surfaces and interfaces, as well as image analysis and processing expertise.

Overview of Services

The SKCC Bioimaging Shared Resource has:

  • A Leica TCS SP8 STED 3X confocal/super-resolution microscope (Leica STED) providing a major step forward in imaging resolution capable of achieving 40 nm lateral and 130 nm axial resolution. Both live cells and fixed samples up to 300 μm thick can be imaged using 3 to 5 different wavelengths. The system also features the LIGHTNING detection package extracting more information from confocal images in real time.

For the new Leica STED system, the Facility will offer up to two hours of assisted use without charge during regular hours for Jefferson researchers to determine if this instrument will be helpful for their projects. Please contact Facility staff for further information.

  • Two point-scanning laser confocal microscopes: (Nikon A1R+) with ultrafast resonant scanning technology and high resolution galvano scanners allowing for high resolution imaging of intracellular dynamic processes. (Nikon C2+) ideal for initial examination of fixed specimens with thicknesses up to several hundred microns.
  • A spinning disk confocal/TIRF microscope (TIRF Microscope) specialized for short-term high-speed observation of live cells, particularly of the cell-substrate interface region.
  • A widefield epifluorescence microscope (Live Cell) for longer-term observation (minutes to hours) of multiple regions of interest in living samples.

Training, Protocol Development & Image Analysis

Our goal is to have all instruments fully operational and ready for usage to accommodate investigators' needs at all times. To this end, the SKCC Bioimaging Shared Resource staff will provide expertise and operate instruments for occasional users who may not need or want to acquire proficiency themselves. For more intensive prospective users, the Resource provides training on the use of all the systems and monitors their usage. Once trained and having satisfactorily demonstrated proficiency to Resource staff, the equipment is available on a 24/7 basis. To ensure that skills are maintained, investigators who have not used the Resource equipment for several months are encouraged to schedule a brief refresher session - this may be required at Resource management discretion.

Advice on protocol development, filter and fluorophore choice is also available. Staff will assist with troubleshooting image quality and data quantification, providing information on applications custom-tailored to the individual investigator's needs.

Publication Acknowledgement

To accurately reflect the assistance the SKCC Bioimaging Shared Resource plays in your research and to help assure its continued funding, please be sure to include the following Resource name and grant number in the acknowledgment section of all publications:

Bioimaging Shared Resource of the Sidney Kimmel Cancer Center (NCI 5 P30 CA-56036), and any staff (as appropriate).

Leadership

James Keen, PhD | SKCC Bioimaging Shared Resource Director

Philip Wedegaertner, PhD | SKCC Bioimaging Shared Resource Co-Director

Maria Yolanda Covarrubias, PhD | SKCC Bioimaging Shared Resource Manager

Location and Hours of Operation

Hours Location

Staffed Monday - Friday, 9am - 5pm          

24/7 access for trained users

 

914 BLSB
233 South 10th Street
Philadelphia, PA 19107

Contacts

Name Role Phone Email Location
Maria Covarrubias, PhD
Facility Manager
 
(215) 503-4770
 
Bioimaging@mail.jci.tju.edu, Maria.Covarrubias@jefferson.edu
 
914 BLSB
 
James Keen, PhD
Facility Director
 
(215) 503-8982
 
james.keen@jefferson.edu
 
912 BLSB
 
Philip Wedegaertner, PhD
Resource Co-Director
 
(215) 503-3137
 
(215) 503-3137
 
839 BLSB
 

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