This facility serves the needs of SKCC research programs in structural biology, including cryo-electron microscopy, crystallization, x-ray data collection, structure determination, and structural analysis of macromolecules. The facility has a ThermoScientific 200 kV Glacios transmission electron microscope equipped with a Falcon 4 direct electron detector, a Vitrobot 5 for cryo-EM sample preparation, a Formulatrix NT8 robot crystallization robot for high-throughput crystallization screening and maintains a Rigaku MicroMax-007HF microfocus rotating anode X-ray generator dual equipped for data collection from macromolecular crystals and Small Angle X-Ray Scattering (SAXS). The left port of this instrument, dedicated to X-ray data collection from macromolecular crystals, is equipped with high focusing mirrors and a new ultrafast PILATUS 200K Hybrid Pixel Array Detector and a X-stream 2000 cryogenic system, while the right port hosts a new BioSAXS-2000 2D Kratky SAXS system.
The facility also provides access to instrumentation for biophysical characterization of proteins, and protein/protein and protein/DNA interactions. These instruments include a Beckman Coulter Analytical Ultracentrifuge (ProteomeLab XL-I/A Protein Characterization System), a JASCO J-810 Spectropolarimeter, a BIACORE 3000 SPR biosensor, and a TA Instruments Nano-Isothermal Titration Calorimeter.
Dmitry Temiakov, Ph.D | Director
Erik Debler, Ph.D. | Co-Director
Ashok Nayak, Ph.D | Cryo-EM Facility Manager
Hideharu Hashimoto, Ph.D. | X-ray Crystallography Facility Manager
Hours | Location |
Staffed Monday - Friday, 9am - 5:00pm 24/7 access for trained users |
Glacios Control room: JAH 444 Vitrobot/ITC: JAH 442 X-ray/CD: JAH 457 1020 Locust Street 19107 Philadelphia PA
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Name | Role | Phone | Location | |
---|---|---|---|---|
Dmitry Temiakov, PhD |
Director
|
(215) 503-4692
|
Dmitry.temiakov@jefferson.edu
|
JAH 425e
|
Erik Debler, PhD |
Co-Director
|
(215) 503-4596
|
Erik.debler@jefferson.edu
|
JAH 411f
|
Ashok Nayak, PhD |
Cryo-EM Manager
|
(215) 503-4030
|
Ashok.nayak@jefferson.edu
|
JAH 425c
|
Hideharu Hashimoto, PhD |
X-ray crystallography Manager
|
(215) 503-9252
|
Hideharu.hashimoto@jefferson.edu
|
JAH 412
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https://goo.gl/maps/QdEEHgUXwXE2