Integrated Structural Biology Shared Resource

Overview of Services

This facility serves the needs of SKCC research programs in structural biology, including cryo-electron microscopy, crystallization, x-ray data collection, structure determination, and structural analysis of macromolecules. The facility has a ThermoScientific 200 kV Glacios transmission electron microscope equipped with a Falcon 4 direct electron detector, a Vitrobot 5 for cryo-EM sample preparation, a Formulatrix NT8 robot crystallization robot for high-throughput crystallization screening and maintains a Rigaku MicroMax-007HF microfocus rotating anode X-ray generator dual equipped for data collection from macromolecular crystals and Small Angle X-Ray Scattering (SAXS). The left port of this instrument, dedicated to X-ray data collection from macromolecular crystals, is equipped with high focusing mirrors and a new ultrafast PILATUS 200K Hybrid Pixel Array Detector and a X-stream 2000 cryogenic system, while the right port hosts a new BioSAXS-2000 2D Kratky SAXS system.

The facility also provides access to instrumentation for biophysical characterization of proteins, and protein/protein and protein/DNA interactions. These instruments include a Beckman Coulter Analytical Ultracentrifuge (ProteomeLab XL-I/A Protein Characterization System), a JASCO J-810 Spectropolarimeter, a BIACORE 3000 SPR biosensor, and a TA Instruments Nano-Isothermal Titration Calorimeter.


Dmitry Temiakov, Ph.D | Director

Erik Debler, Ph.D. | Co-Director

Ashok Nayak, Ph.D | Cryo-EM Facility Manager

Hideharu Hashimoto, Ph.D. | X-ray Crystallography Facility Manager

Location and hours of operation

Hours Location

Staffed Monday - Friday, 9am - 5:00pm          

24/7 access for trained users

Glacios Control room: JAH 444

Vitrobot/ITC: JAH 442

X-ray/CD: JAH 457

1020 Locust Street

19107 Philadelphia PA


Links and Resources


Name Role Phone Email Location
Dmitry Temiakov, PhD
(215) 503-4692
JAH 425e
Erik Debler, PhD
(215) 503-4596
JAH 411f
Ashok Nayak, PhD
Cryo-EM Manager
(215) 503-4030
JAH 425c
Hideharu Hashimoto, PhD
X-ray crystallography Manager
(215) 503-9252
JAH 412