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X-ray Crystallography and Molecular Characterization Shared Resource

Overview of Services

This facility serves the needs of SKCC research programs in crystallization, X-ray data collection, structure determination, refinement and structural analysis of macromolecules. The facility has HydraIIPlusOne and Oryx8 crystallization robots for high-throughput crystallization screening and maintains Rigaku MicroMax-007HF microfocus rotating anode X-ray generator dual equipped for data collection from macromolecular crystals and Small Angle X-Ray Scattering (SAXS). The left port of this instrument, dedicated to X-ray data collection from macromolecular crystals, is equipped with high focusing mirrors and a new ultrafast PILATUS 200K Hybrid Pixel Array Detector and a X-stream 2000 cryogenic system, while the right port hosts a new BioSAXS-2000 2D Kratky SAXS system.

The facility also provides access to instrumentation for biophysical characterization of proteins, and protein/protein and protein/DNA interactions. These instruments include a Beckman Coulter Analytical Ultracentrifuge (ProteomeLab XL-I/A Protein Characterization System), a JASCO J-810 Spectropolarimeter, a BIACORE 3000 SPR biosensor, a KinExA 3000, and two Isothermal Titration Calorimeters: MicroCal-ITC and a TA Instruments Nano-ITC.

Leadership

Gino Cingolani, Ph.D | Director, X-ray Crystallography

Marzia Niazi, M.S. | Facility Manager, X-ray Crystallography and Molecular Characterization Shared Resource

Location and hours of operation

Hours Location

Staffed Monday - Friday, 9am - 5:00pm          

24/7 access for trained users

JAH-4E, Rm 457 

1020 Locust Street

19107 Philadelphia PA

 

Links and Resources

Contacts

Name Role Phone Email Location
Gino Cingolani, Ph.D.
Director
 
2155034587
 
gino.cingolani@jefferson.edu
 
411K JAH
 
Marzia Niazi, M.S.
Manager
 
2155034587
 
marzia.niazi@jefferson.edu
 
4E-457 JAH
 

Map


https://goo.gl/maps/QdEEHgUXwXE2