Flow Cytometry Facility

"ALL samples brought to our facility should be filtered with 70-micron cell strainer"

Overview of Services

The Sidney Kimmel Cancer Center facility provides state-of-the-art fluorescence activated cell sorting and analysis capabilities to investigators of the Sidney Kimmel Cancer Center and the Philadelphia region. The most commonly used applications are multi-color cell surface phenotyping, detection of intracellular cytokines and signaling molecules, transfection measurements, studies of apoptosis, cell cycle analysis, cell proliferation, and real time analysis of Ca2+ mobilization kinetics. The sorters, analyzers, and workstations allow for affordable, reliable, and accurate characterization of a wide variety of biological samples.

The services provided by the facility can be tailored to suit experimental designs in a variety of fields. We provide consultation on current protocols, analysis techniques, and data presentation. This resource plays a critical role in assisting SKCC members performing various research projects directly related to the understanding of the biology and treatment of cancer. Furthermore the facility supports projects from investigators in Biochemistry, Genetics, Immunology, Medicine, Microbiology, Molecular Biology, Neurology, Pathology, Pharmacology, Surgery, and many other scientific fields. The facility current has 5-laser-16-color BD AriaII high speed sorter, 5-laser-16-color BD Fortessa, 4-laser-14-color BD LSRII.

Leadership

Jianke Zhang, PhD
Director
jianke.zhang@jefferson.edu

Staff

Lei Yu, PhD
Manager
215.503.3216
lei.yu@jefferson.edu

 

Amir Yarmahmoodi, BSc
Technician
215.503.4556
amir.yarmahmoodi@jefferson.edu 

Hours of Operation and Location

Hours Location

Monday - Friday

9:00 AM-5:00PM                        

233 S 10th Street, Room 606
Philadelphia, PA 19107

 

Links and Resources

  1. FluoroFinder
  2. Flow Cytometry Shared Resource

Contacts

Name Role Phone Email Location
Lei Yu, Ph. D.
Manager
 
215.503.3216
 
lei.yu@jefferson.edu
 
BLSB 606A
 

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