Translational Pathology Shared Resource

Note: TPSR users, please remember to acknowledge use of TPSR in publications: "We acknowledge the use of the Translational Pathology Shared Resource of the Sidney Kimmel Comprehensive Cancer Center, Thomas Jefferson University, to conduct this research (supported by NCI grant 5P30CA056036) - OR - "We acknowledge the following Shared Resources at Sidney Kimmel Comprehensive Cancer Center, Thomas Jefferson University, that were used to conduct this research: Translational Pathology, X, and Y Shared Resources (supported by NCI grant 5P30CA056036)".

Note: New employee: Please welcome our new Research Assistant, Naheed Perez, MS. Naheed recently joined TPSR after completing her Master's degree at the Philadelphia College of Osteopathic Medicine (PCOM), Philadelphia, PA.

Note: Tissue Processors Upgraded: TPSR has replaced the two old Thermo tissue processors with two new Thermo Excelsior tissue processors. 

Note: Slide-Scanner Upgraded: TPSR has replaced its 5-slide Leica Aperio CS2 with a 1,000-slide 3DHISTECH Pannoramic P1000 at no added cost to users. For SKCCC members, standard 20× scans are $5 per slide, falling to $4.75 (101–200 slides), $4.50 (201–300), $4.25 (301–400), and $4.00 (401+). High-resolution 40× scans are $10 per slide with the same discount tiers: $9.50, $9.00, $8.50, and $8.00.

Note: Immunohistochemistry Services Upgraded: New Ventana Discover Ultra Autostainer: TPSR has replaced the Biocare autostainer with the Ventana Discovery system, delivering sharper signals and lower background via polymer‑based amplification (see Fig. 1). A service cost increase from $31 to $34 per slide (SKCCC members) has been implemented to cover higher reagent expenses. This upgrade was funded by Dr. Haifeng Yang’s S10 grant, which many Jefferson and Drexel faculty members graciously supported.

 

TPSR Sample Drop-Off and Pick-Up Procedures: 

For routine requests submitted through iLab, walk-in drop-offs/pick-ups are welcome during our work hours (Mon-Fri, 8:30 am to 4:30 pm). 

For consultation requests, please schedule an appointment by calling 215-503-3858/215-503-8826 or Emailing natalie.dougherty@jefferson.edu. 

For further assistance, please refer to our Useful Links and Resources below.

When dropping off:

• Please label the sample container(s)/package with the request ID and the client's name.
• Please print a hard copy of your request sheet and submit it with the other materials.
• Please talk to one of our staff when dropping off samples so we can check the samples and request details, safely store/secure the submitted materials, and verify and update your request status.

Click here for Full Procedures to Utilize this Shared Resource 

 

 

Our Instruments

Work Offered

1. Full range of experimental histopathology services including paraffin and frozen tissue processing, slide preparation, histochemical staining (H&E and Special Stains)

2. Immunohistochemistry (IHC) staining is performed on paraffin sections, frozen tissue, or cytological preparations. It utilizes a broad range of optimized commercial antibodies or antibodies provided by the investigator with an automatic staining platform (Ventana Discovery Ultra)

3. Bright-field whole slide scanning with the high capacity 3DHistech P1000 or with the Aperio CS2 ScanScope (Link to our Image server: SKCC TPSR eSlideManager Server Link)

Useful Links and Resources

How to Use iLab:

1. iLab user tutorials videos

2. How to manage membership request and charge codes(for PI)

Preparing your Samples:

1. Sample preparation instruction for paraffin embedding

2. Sample preparation instruction for frozen embedding

3. Preparing Gel Block from Cell Lines or Cell Suspensions

Slide Scanner Software & Tutorials:

1. Aperio eSlideManager Server

2. Policy for Aperio CS2 Digital Slide Scanning and Access of the eSlide Manager

3. Aperio eSlide Manager User Account Request Form 

4. Aperio CS2 Digital Image Scanning Sample Information Collection Form

5. Aperio eSlideManager Operator Guide

6. Aperio ImageScope User Guide

7. Aperio WebScope User Guide

8. Download SlideViewer Software for P1000 Scanner

9. SlideViewer User Guide

Sample Preparation:

1. Revised guides for organ sampling and trimming in rats and mice: Parts 1 – 3(A joint publication of the RITA and NACAD groups)

2. The Internet Pathology Laboratory for Medical Education (Hosted By University of Utah Eccles Health Sciences Library)

Choosing Antibodies:

1. Selecting the Optimal Antibody

2. The Human Protein Atlas

3. CiteAb

 

Leadership

Natalie Dougherty, MS | Laboratory Manager

Haifeng Yang, PhD | Co-Director

Hallgeir Rui, MD, PhD | Director

 

Address: 

233 S. 10th Street
Room 815, Bluemle Life Sciences Building       
Philadelphia, PA 19107

Contacts

Name	Role	Phone	Email	Location
Hallgeir Rui, MD, PhD	Director	(215) 503-9881	hallgeir.rui@jefferson.edu	Bluemle Life Sciences Building, Rm 1008
Haifeng Yang, PhD	Co-Director	(215) 503-6163	haifeng.yang@jefferson.edu	Jefferson Alumni Hall, 336D
Natalie Dougherty, MS	Manager	(215) 503-3858/8826/2237	natalie.dougherty@jefferson.edu	Bluemle Life Sciences Building, Rm 815
Ruiyan Jing, BA	Research Assistant Senior	(215) 503-3858/8826/2237	ruiyan.jing@jefferson.edu	Bluemle Life Sciences Building, Rm 815
Naheed Perez, MS	Research Assistant Intermediate	(215) 503-3858	naheed.perez@jefferson.edu	Bluemle Life Sciences Building, Rm 1008
Linna Ge	Lab Specialist		linna.ge@jefferson.edu	Bluemle Life Sciences Building, Rm 815
Yunguang Sun, MD, PhD	Digital Pathology	(215) 503-4673	yunguang.sun@jefferson.edu	Bluemle Life Sciences Building, Rm 1020

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