Translational Research/Pathology Shared Resource

TR/PSR Social Distancing Procedures:

According to TJU General Social Distancing Guidelines,  please follow the  procedures below to submit/retrieve materials to this shared resource and/or set up a consultation meeting to keep both our staff and clients safe:

1.     No contact samples drop-off:

a. For all approved projects, customers must schedule material drop-off time via telephone:

   215-503-3858/215-503-8826

b. There will be a designated table outside the lab in the hallway for drop-off.

c. Sample containers must be labelled with the request ID and/or name of the customer.

d. Hardcopy of request sheet is not required during the epidemic.

e. Our staff will pick up the material ASAP and decontaminate the containers and the table.

2.     No contact samples retrieval:

a. For completed project, customers must schedule pick-up time via telephone:

    215-503-3858/215-503-8826

b.  Our staff will decontaminate exterior of finished work before putting them outside for retrieval.

c.  Finished work will be left on a designated table outside the lab in the hallway before the scheduled time.

3. Remote Consultation Service: 

We will provide remote consultation through telephone or Zoom Meeting. 

4. Utilization of Cryostat is suspended:

This service is temporary suspended until the situation is allowed. All approved frozen section projects will be completed by staff of this shared resource.

About us:

The Translational Research/Pathology Shared Resource provides full range, state of the art, detail orientated, outstanding array of experimental histopathology services complemented by molecular pathology, digital pathology and veterinary hematology services to SKCC members, TJU and local research community in greater Philadelphia area. In collaboration with our internal labs at TJU, we also facilitate the interactions between investigators and SKCC Biorepository and Pathology Department to access human tissue samples and perform Laser Capture Microdissection of tissues to capture individual cells. The Translational Research/Pathology Shared Resource is used by over 100 principle investigators and has provided key data that support many publications and grant applications.

Our Instruments

Mission

The mission of this shared resource is to provide:

1. Full range outstanding array of experimental histopathology services, including paraffin and frozen tissue processing, slide preparation, histochemical staining (H&E and Special Stains)

2. Immunohistochemistry (IHC) staining, which is performed on paraffin sections, frozen tissue or cytological preparations and utilizes a broad range of optimized commercial antibodies or antibodies provided by the investigator with automatic staining platform: DAKO Autostainer Plus and Biocare Medical intelliPATH FLX®.

3. TUNEL Labelling and in situ Hybridization using RNAscope® technique

4. Light field whole slides scanning (20X and 40X) with Aperio CS2 ScanScope.

5. Quantitative, automated veterinary Complete Blood Count (CBC) using GENESIS Veterinary Hematology Analyzer.

Procedures to Utilize This Shared Resource:

For all first time users, please watch iLab user tutorials videos.

The procedures are:

1. Both lab member who is going to use SKCC shared resources under iLab and their PI need register in iLab system if they haven’t done so. For internal users, they will use their Jefferson campus key and password to log in after complete the registration. External users can generate their own user name and password. PI should register first, so their lab member can register under the PI’s lab. PI need approve the lab member’s membership in iLab system before he can join the lab in iLab system. See instruction How to manage membership request and charge codes (for PI) 

See iLab registration link: https://skcc-pa.ilabsolutions.com/landing/2792

2. For internal users, PI need set up payment information with our IT expert Ryan Smalley (Tel: 3-9692, Email: Ryan.Smalley@jefferson.edu) after the registration. 

    For external users, please generate a PO(Purchase Order) before submit request to us.

    No request will go through the iLab system to us without payment information.

3. For internal users, PI need assign charge code to their lab member to allow them to access the charge code. See instruction How to manage membership request and charge codes (for PI)

4. After PI assigns the charge code, the lab member can submit a request to us in iLab system under Request Service tab. Please fill out samples information and request details in the requisition section and print out the request sheet (use the printer icon under "Forms and Request Details" to download a PDF file and print it out).

5. For non-Frozen request, no approval is needed. Please submit the request sheet together with the samples to us. For frozen request, please always call 215-503-3858/8826 or email us to make an appointment before submitting the samples to this shared resource.

6. We will process the request once we received the hard copy of the request sheet and samples. An automatic email(from: no-reply@ilabsolutions.com titled "NOTIFICATION: XXXX The Service request TR/PSR-XX-XXXX has been completed") will be sent to you once we update the request status to "completed".

7. You can come and bring you final work back to your lab.

Leadership

Wei Jiang, MD, PhD | Director

Haifeng Yang, PhD | Co-Director

Hours of Operation, Location and Telephone

Useful Links and Resources

1. iLab user tutorials videos

2. How to manage membership request and charge codes(for PI)

3. SKCC Translational Research/Pathology Shared Resource - List of Services & Price Schedule

4. Sidney Kimmel Cancer Center Shared Resources

5. Sample preparation instruction for paraffin embedding

6. Sample preparation instruction for frozen embedding

7. Preparing Gel Block from Cell Lines or Cell Suspensions

8. Automatic Animal Complete Blood Count (CBC) Customer Instruction

9. Policy for Aperio CS2 Digital Slide Scanning and Access of the eSlide Manager

10. Aperio eSlide Manager User Account Request Form 

11. Aperio CS2 Digital Image Scanning Sample Information Collection Form

12. Aperio eSlideManager Operator Guide

13. Aperio ImageScope User Guide

14. Aperio WebScope User Guide

15. Revised guides for organ sampling and trimming in rats and mice: Parts 1 – 3(A joint publication of the RITA and NACAD groups)

16. The Internet Pathology Laboratory for Medical Education (Hosted By The University of Utah Eccles Health Sciences Library)

Contacts

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